ReadyMedical

EFFICACY TESTS

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REVITALIZING: HA+GF

Human fibroblasts have been treated in vitro with ReadyMedical special compound of sonicated Hyaluronic Acid + Peptides with the wound healing technique. After 24 hours the treated cells had increased their motility and proliferation of up to 44%, compared to the untreated cells used as negative controls (1).

Using the inserts, a gap of 500 μm was created in a confluent cell colture monolayer and the wound closure was evaluated by measuring the width of injury line, taking pictures at regular interval of 24 hours.

The effect of the Product ReadyMedical HA + GF was evaluated as the capability of cells to reduce the original gap of 500 μm (100% of wound closure), comparing the pictures, taken at different times with the corresponding pictures of the initial wounded monolayer. The result has been the complete wound closure of the cells treated with ReadyMedical HA + GF after 72 hours.

1) UB-CARE – UNIVERSITY OF PAVIA. Report nr. PRL/17/LC1/P2. Date: 25/07/2017. The full version of this study is available upon request.

ACTIVE INGREDIENTS CONTAINED IN THE 0,5 ML GF SYRINGE

Copper Tripeptide-1: The molecular structure of copper tripeptide-1 is indicated by its name, i.e. a tripeptide chain consisting of glycine, histidine, and lysine (GHK) complexed with copper. Copper tripeptide-1 is a growth factor composed of only three amino acids (a tripeptide).
Copper tripeptide-1 is a novel compound of great interest to aesthetics. This is related to its ubiquitous abilities not only in skin but also in other tissues to initiate tissue regeneration and wound repair for all types of wounds, increase collagen synthesis, effect DNA repair, quiet excess inflammatory processes, and acts as an antioxidant. For these reasons it is used for improving post-procedure results and decreasing the velocity of ageing is obvious. Its safety profile is also quite remarkable and increases the level of interest. No adverse effects or issues have been found for copper-tripeptide-1 in spite of extensive research involving the compound since the early 1980s. As well as acting as a growth factor, it also has attractive anti-neoplastic effects that substantiate its safety profile for the ageing patient.

Action: SKIN PENETRATION Copper tripeptide-1 easily penetrates the stratum corneum barrier.

Action: WOUND HEALING A huge body of scientific evidence supports the essential role of copper tripeptide-1 in the acceleration of wound healing in many human and animal wound types, including surgical, post-laser, Mohs, ischemic, burns, large skin grafts, hair transplants, refractory venous stasis ulcers, diabetic ulcers, and others. Diabetic wounds healed three times faster in the presence of copper tripeptide-1 and time to re-epithelialization was shortened.

Action: TISSUE REMODELLING AND SKIN RENEWAL Copper tripeptide-1 is active not only in wound healing but also for normal tissue remodelling. Studies have documented these regenerative effects in both human and animal studies. In skin, the specific effects of copper tripeptide-1 include increasing keratinocyte proliferation and normal collagen synthesis, improving skin thickness, skin elasticity and firmness, improving wrinkles, photodamage and glycation, uneven pigmentation, skin clarity and tightening, and upregulating protective barrier proteins.

Sh-Polypeptide-11: It is an acidic Fibroblast Growth Factor that modulates the skin rejuvenating by stimulation of cell proliferation. It strengthens skin elasticity by inducing the synthesis of collagen and elastin. Has healing and wound repair properties.

Sh-Polypeptide-3: It is a Keratinocyte Growth Factor (CG-KGF) that is able to reduce and prevent lines and wrinkles by actively generating new skin cells, to refine texture glides effectively and light up skin. KGF protects keratinocytes from UV cell damage.

Sh-Oligopeptide-2: It is a insulin-like Growth Factor that modulates the skin rejuvenating by stimulation of cell proliferation. It reduces and prevents lines and wrinkles by actively generating new skin cells. Increases collagen and elastin levels and reduces blotchiness. It refines texture glides effectively and slims your face (and body) with a fat burning effect.

Sh-Polypeptide-1: It is a basic Fibroblast Growth Factor (CG-bFGF) that reduces and prevents lines and wrinkles by actively generating new skin cells. It helps in normal skin growth, healing and repairing wounds. It strengthens skin elasticity by inducing the synthesis of collagen and elastin. Sh-Oligopeptide-1 It is an Epidermal Growth Factor (CG-EGF) that reduces and prevents lines and wrinkles by actively generating new cells, enhances tone for skin that is brimming with vitality and energy, nourishes skin that appears smoother, brighter and regains its youthful look. Finally it eliminates scars by forming new skin cells.

INGREDIENTS

POST-TREATMENT: LENITIVE HA+PFD+PA

The study has been conducted through the in vitro evaluation of the anti-inflammatory activity of ReadyMedical HA+PFD+PA (1) on human fibroblasts.
A human keratinocyte cell line has been treated with this special compound of sonicated Hyaluronic Acid + Perfluorodecalin + Physalis Angulata. The treated cells showed a decrease of ~ 94% of inflammation compared to the untreated cells used as negative control.
The anti-inflammatory activity has been calculated measuring the IL-8 levels 24 hours after treating the fibroblasts with the product; the result was a decrease of the IL-8 levels between 93,25% and 94,82%, compared to the untreated cells.
IL-8 is the most direct and reliable marker of inflammation among the wide cytokine family. Cytokines are small proteins known to play an important role in the immune system; in particular they are able to influence the activation, maturation, growth and differentiation of several cell populations. IL-8 secretion is particularly active in chronic inflammation conditions, thus measurement of this cytokine in an in vitro model, composed by fibroblasts, represents a useful and sensitive tool to assess the anti-inflammatory effect of a topical product.

1) UB-CARE – UNIVERSITY OF PAVIA. Report nr. AIF/17/LC1/P1. Date: 12/07/2017. The full version of this study is available upon request.

ACTIVE INGREDIENTS CONTAINED IN THE 0,5 ML GF SYRINGE

Perfluorodecalin It is a fluorocarbon and a derivative of decalin that can carry and bind up to 49% of its volume with oxygen. Increasing the oxygen levels PFD significantly provides faster wound healing and reduced erithema and scarring. Several studies(5) demonstrate that the application of oxygen activates the inflammatory cells of the immune system that help healing.

As demonstrated by studies(6), during Q-switched laser treatments for tattoo removal, topical PFD is able to rapidly resolve clinical withening and permit transmission of light within few seconds (5 seconds – range 3-10 seconds) of application, resulting in effective multi-pass tattoo fading. Using multiple passes per session, with each pass delivered after resolution of whitening, improves tattoo fading in a 60 minute treatment time.
In addition to clearance of micro-bubbles that limit light penetration, perfluorocarbon properties of optical clarity, may also indipendently increase optical penetration of light in PFD-treated skin. PFD saturates air spaces well when applied to a surface and may increase the depth of penetration of laser treatment. PFD is to be considered an effective anti-aging active ingredient too, as it remarkably improves skin moisturization, thus resulting in anti-wrinkle effects and younger, instant smooth and soft looking skin.

Physalis Angulata is a vegetable-origin active ingredient. It is extremely efficacious as corticoid-like due to its immense immunomodulating and its ‘anti-photo-aging’ properties. Further, it increases serine proteases, maintaining and repairing skin collagen.
After in vitro tests that compared it with hydrocortisone, PA was shown to have similar characteristics (decreasing pro-inflammatory cytokines and inflammatory mediators and related enzymes) but not the collateral effects common to cortico-steroids, such as immunosuppression or reduced tissue growth factor (TGF).
PA decreases intradermical microinflammation, regulates intradermal temperature, providing protection against infrared rays and visible light and increases collagen.

INGREDIENTS

ACNYCID: HA + AC

STUDY 1
In vitro EVALUATION OF THE PROPIONIBACTERIUM ACNES GROWTH INHIBITION AFTER THE TREATMENT
WITH READYMEDICAL ACNYCID HA + AC
The inhibitory activity of the ReadyMedical Acnycid product was evaluated by treating the Propionibacterium acnes bacterial strain with the tested product. At two time intervals (6h and 24h), 100 μl of the mixture were plated on selective agar plates in order to verify the possible inhibition of the bacterial growth of Propionibacterium acnes, represented in Figure.
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The results obtained show that the ReadyMedical Acnycid product determines a decrease equal to 99.9% of the growth of the Propionibacterium acnes strain after 6 hours and after 24 hours of contact, which was highly significant under the conditions tested.

Figure: Growth inhibition of P. acnes in absence (Inoculum ctrl) and presence (100%) of the ReadyMedical Acnycid product after different times of contact.
STUDY 2
In vitro EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF READY MEDICAL ACNYCID HA + AC AFTER PROPIONIBACTERIUM ACNES STIMULUS ON HUMAN KERATINOCYTES
The anti-inflammatory activity of the product ReadyMedical Acnycid after stimulation with P.acnes was evaluated in HaCat cells, measuring the expression of IL-8 by an ELISA kit (2). The concentrations to be used for the anti-inflammatory assay are 56.2 and 46.4 mg/ml, based on the cell viability assay. Positive control is represented by untreated cells and stimulated with P.acnes colture.
The obtained results demonstrate a decrease in IL-8 levels, following a pro-inflammatory stimulus, in human keratinocytes treated for 8 h. Samples treated with the product ReadyMedical Acnycid show a decrease in IL-8 levels of 39.39% and 41.69% (following treatment with 56.42 and 46.4 mg/ml, respectively) compared to the stimulated control (Ctrl + P.acnes), resulted to be highly significant for both tested concentrations.

Figure: Shows the effect of 8 h treatment with the test- ed product on IL-8 production, after P. acnes stimulation.

ACTIVE INGREDIENTS CONTAINED IN THE 0,5 ML GF SYRINGE

10-Hydroxydecanoic Acid, Sebacic Acid, 1,10-Decanediol Royal Jelly mimetic that helps balance sebum. This is a unique active ingredient aimed at re- establishing the physiological balance in acne-prone skin. It is composed of 10-hydroxydecanoic acid (10-HDA), sebacic acid and decanediol. The target molecule, 10-HDA, is a synthetic bio-mimetic version of a hydroxyl acid naturally present in Royal Jelly. This mix of active ingredients help the seboregulation and have an anti-bacterial effect.

Aloe Vera Gel (Aloe Barbadensis Leaf Juice) Aloe Vera contains healing and antiseptic compounds that help to heal and prevent acne. The glucomannan contained is a mannose-rich polysaccharide that works as an antiseptic. Aloe vera works as an astringent on the skin, cleaning it, and clearing out pores of sebum, dirt and dead skin cells.

Heptapeptide-10 This active ingredient has soothing and calming properties, acting as an anti-histamine or immune suppressor helps to reduce scratching by inhibiting the autoimmune response.

Palmitoyl Tripeptide-8 This is a true neurocosmetic specifically designed to prevent and reverse signs of neurogenic inflammation (redness and swelling). It is an innovative biomimetic tri-peptide derived from neuromediator (POMC) linked to a lipid for optimal penetration and efficacy. Palmitoyl Tripeptide-8 is a multifunctional active that calms and soothes irritated skin.

Nettle (Hydrolyzed Nettle Leaf Extract) Innovative and selective vegetable extract of nettle leaves (Urtica dioica). It contains the concentrated protein fraction, enzymatically hydrolyzed, with free amino acids present and part of the water-soluble principles of the plant. It has a hydrating, soothing and purifying action.

Phitic Acid Phytic Acid is used in dermatology for its lenitive, anti-tyrosinase, anti-oxidant, whitening and chelating properties.

INGREDIENTS
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ReadyMedical is a registered trademark of :

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ReadyMedical is the first professional line of ready to mix sterile cosmetic products specifically created for Physicians and for pratictioners in the field of aesthetics.

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Jet Tech Europe Srl
Via G. Silvestri, 15
20126 Milano (Italy)
Phone: +39 02 954 205 385
Email: info@readymedical.it

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